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マトリックスメタロプロテイナーゼ（MMP）アッセイシステム

代表的な測定マーカー：MMP、TIMP　

製品 コード番号 原理 包装 アッセイレンジ 感度 操作時間 備考
MMP-1, Human RPN2610 ELISA 96 wells 6.25～100 ng/ml

1.7 ng/ml 6.5 hr.
  • Specific for total MMP-1 （proMMP-1, active MMP-1 & MMP-1 / TIMP-1 complex）
  • Suitable for use with cell culture supernatant and serum / plasma samples
  • Sample volume: 100 μl
MMP-1 Activity Assay 製造終了品 RPN2629 MMP Activity Assay 96 wells 3.13～50 ng/ml
（1.5 hr. incubation with detection reagent）

0.1～1.56 ng/ml
（4.0 hr. incubation with detection reagent）

2.2 ng/ml

0.1 ng/ml

 1.5 days
  • Measures endogenous active MMP-1 or total active MMP-1
  • Range / sensitivity: Equivalent to ELISA
  • Ease-of-use: Equivalent to ELISA
MMP-2, Human RPN2617 ELISA 96 wells 1.5～24 ng/ml 0.37 ng/ml 4.5 hr.
  • Specific for proMMP-2
    （free and complexed to TIMP-2）, but not the active form of MMP-2
  • Suitable for use with cell culture supernatant, serum / plasma and tissue samples
  • Sample volume: 100 μl
MMP-2 Activity Assay RPN2631 MMP Activity Assay 96 wells 0.19～3.0 ng/ml
（4.0 hr. incubation with detection reagent）

0.75～12 ng/ml
（1.5 hr. incubation with detection reagent）		 0.19 ng/ml

0.5 ng/ml		 1.5 days
  • Suitable for use with cell culture supernatant, serum and plasma samples
  • Measures either endogenous （naturally occurring）or total active MMP-2（activated by APMA）
  • Sensitivity equivalent to ELISA / Zymography
  • Ease-of-use: Equivalent to ELISA
  • Sample volume: 100 μl
MMP-3 Activity Assay 製造終了品 RPN2639 MMP Activity Assay 96 wells 0.5～32 ng/ml
（1.5～2.5 hr. incubation with detection reagent）

0.125～8.0 ng/ml
（3.0～4.0 hr. incubation with detection reagent）		 0.5 ng/ml

0.1 ng/ml		 1.5 days
  • Measures either endogenous active MMP-3 （naturally occurring） or total active MMP-3 （following activation with APMA）
  • Quantitative
MMP-7, Human 製造終了品 RPN2620 ELISA 96 wells 0.31～10 ng/ml 97 pg/ml 1.5 days
  • Specific for human proMMP-7
  • Suitable for use with cell culture supernatants
  • Sample volume: 100 μl
MMP-8, Human RPN2619 ELISA 96 wells 0.25～4.0 ng/ml 32 pg/ml 4.5 hr.
  • Specific for:
    pro- and active MMP-8: 100%
    active MMP-8/ TIMP-1 complex: 50%
    active MMP-8/ TIMP-2 complex: 30%
  • Suitable for use with cell culture supernatant, serum / plasma and tissue samples
  • Sample volume: 100 μl
MMP-8 Activity Assay 製造終了品 RPN2635 MMP Activity Assay 96 wells 0.75～24 ng/ml
（2.0 hr. incubation with detection reagent）

0.09～6.0 ng/ml
（3.0～4.0 hr. incubation with detection reagent）		 0.1 ng/ml 1.5 days
  • Measures either endogenous active MMP-8 or total active MMP-8 （activated by APMA）
  • Quantitative
MMP-9, Human RPN2614 ELISA 96 wells 1.0～32 ng/ml
（cell culture supernatant）

4.0～128 ng/ml
（plasma sample）		 0.6 ng/ml 4.5 hr.
（cell culture supernatant）

2.5 hr. （plasma sample）
  • Specific for proMMP-9 （free and complexed to TIMP-1, TIMP-2）
  • Suitable for use with cell culture supernatant and plasma samples
  • Sample volume: 100 μl
MMP-9 Activity Assay RPN2634 MMP Activity Assay 96 wells 0.5～16 ng/ml
（1.0 hr. incubation with detection reagent）

0.125～4.0 ng/ml
（1.5 hr. incubation with detection reagent）
 0.125 pg/ml 1.5 days
  • Measures endogenous active MMP-9 or total active MMP-9
  • Range / sensitivity: Equivalent to ELISA
  • Ease-of-use: Equivalent to ELISA
MMP-13, Human RPN2621 ELISA 96 wells 0.094～3.0 ng/ml 0.032 ng/ml 4.0 hr.
  • Specific for human pro- and active MMP-13
  • Suitable for use with cell culture supernatant, serum and plasma samples
  • Sample volume: 100 μl
MMP-14 Activity Assay 製造終了品 RPN2637 MMP Activity Assay 96 wells

1.0～32 ng/ml
（2.5 hr. incubation with detection reagent）

0.125～4.0 ng/ml
（6.0 hr. incubation with detection reagent）

 0.7 ng/ml 1.5 days
  • Measures endogenous active MMP-14 （naturally occurring） in complex biological samples
  • Quantitative
TIMP-1, Human 製造終了品 RPNJ409 ELISA 96 wells 51～2,000 ng/ml 1.2 ng/ml 2.0 hr.
  • Suitable for use with urine, serum, plasma and synovial fluid.
  • Sample volume: 10 μl
  • 日本語取扱説明書添付
TIMP-2, Human RPN2618 ELISA 96 wells 8.0～128 ng/ml 3.0 ng/ml 3.5 hr.
  • Specific for free cell TIMP-2 and TIMP-2 complexed with active forms of MMPs, but not TIMP-2 complexed with pro-MMP-2 or to free TIMP-1 or free TIMP-3
  • Suitable for use with cell culture supernatant and serum / plasma and tissue samples
  • Sample volume: 100 μl


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